Naïve Library Screening Services
BDS has made two naive libraries that we can screen against your target of choice. We have a human scFv library made from the cDNA of over a hundred donors with a diversity of approximately 2 X 109. BDS has also built a naive VHH library from over ten llamas with a diversity of approximately 1 X 109. Screening of a naive library is relatively rapid and less expensive than an immunized library but the diversity and affinity of the clones is usually less than those from an immunized library. We do have the ability to perform affinity maturation to improve affinity and specificity of selected clones. After the rounds of selection, we can deliver sequence information of unique clones and/or small quantities of purified, soluble antibody.
Immunized Library Creation and Screening
BDS specializes in the phage display library creation of antibodies, obtained from immunized sources. Our most common requests are VHH single domain antibody libraries from immunized alpacas or llamas. We can assist with sourcing of antigen and immunization strategy as well as the library creation and phage display library screening. The deliverables can be simple sequence information of the selected antibodies or small quantities of soluble, purified antibody.
Our phage display vector and display system is unique and has a number of advantageous properties.
We have also made custom scFv libraries from mouse and human and can do a variety of other sources (rabbit, sheep, bovine) as long as antibody sequence information is available.
We can build the binding domain into an Fc backbone or other configuration and can do a variety of optimization procedures if necessary.