Antibody Expression of scFv and VHHs
We can prepare antibody expression for VHH and scFv antibodies in E. coli and mammalian cells, and the antibodies will have both an N-terminal FLAG and C-terminal HIS tag. The yield will average 100-200 ug for small-scale production (usually higher for VHH) that can be scaled up for increased yields. The antibodies are purified via the HIS tag by Ni-NTA chromatography or using the FLAGG tag and generally are about 90%+ pure. We can also incorporate alternative tags.
Both scFvs and VHHs can be engineered into a traditional antibody format (see Antibody Engineering for more information) by fusing onto a Fc backbone. Antibody generation produces these antibodies at small scale in mammalian expression systems with resulting yields of 200-400 ug and also are purified via protein A.
We can readily scale up expression or increase yield of antibodies if desired.
Learn More About Our Antibody Generation Services Today
Contact our team at Biomolecular Discovery Services to learn more about our antibody engineering and generation services. We'll be happy to answer any questions you have about our processes and VHH libraries.
