Antibody Expression of scFv and VHHs
Our standard mode of expression for VHH and scFv antibodies is in E. coli and the antibodies will have both a N-terminal FLAG and C-terminal HIS tag. Yield will average 100-200 ug for small-scale production (usually higher for VHH) that can be scaled up for increased yields. The antibodies are purified via the HIS tag by Ni-NTA chromatography and generally are about 90% pure. Both scFvs and VHHs can be engineered into a traditional antibody format (see Antibody Engineering) by fusing onto a Fc backbone. These antibodies are then produced at small scale in mammalian expression system with resulting yields of 100-200 ug and also are purified via the HIS tag.