Improving Antibody Affinity
One of the most common problems with an antibody that has the desired specificity is that the affinity is not sufficient. It is not uncommon for antibodies selected from Naïve libraries to require affinity maturation. However, existing antibodies can also have their affinity improved by this approach as well. A specific library is typically made of the antibody of interest with random mutations, some of which will result in improved affinity. The library is selected under appropriate conditions and the resulting molecules are further characterized for their resulting affinity compared to the parent molecule. Specific regions can also be targeted for mutagenesis as well.